| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Chlorobium limicola | TCCACAACAGCGGAGAACC | AAAACCAAGCACCGCACC | 59.93 | 59.19 | 224 | 182 |
100.00%
|
100.00%
|
| Primer 2 | Chlorobium limicola | ACGATCCACAACAGCGGAG | AAAACCAAGCACCGCACC | 60.08 | 59.19 | 228 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Chlorobium limicola | CACCGTCAGCCAATCCGAT | AAAACCAAGCACCGCACC | 60.15 | 59.19 | 194 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Chlorobium limicola | ACCGTCAGCCAATCCGAT | AAAACCAAGCACCGCACC | 58.69 | 59.19 | 193 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Chlorobium limicola | ACGATCCACAACAGCGGA | AAAACCAAGCACCGCACC | 58.94 | 59.19 | 228 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Chlorobium limicola | AACGATCCACAACAGCGGA | AAAACCAAGCACCGCACC | 59.63 | 59.19 | 229 | 140 |
100.00%
|
100.00%
|
| Primer 7 | Chlorobium limicola | CGTTTTGCGTGGACTGCA | GCAAGTTCGCGATCGTTCC | 59.29 | 59.94 | 294 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Chlorobium limicola | CGTTTTGCGTGGACTGCA | GGCATCATGATCGGGAGCA | 59.29 | 59.93 | 265 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Chlorobium limicola | CGTTTTGCGTGGACTGCA | TACGCTCCGCACTCAACTC | 59.29 | 59.79 | 155 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Chlorobium limicola | CGGAACAGAATGCTGCAGC | TGCTCAATGGCCTGCACT | 59.86 | 59.56 | 222 | 138 |
100.00%
|
100.00%
|